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author | adam <adam@pkgsrc.org> | 2018-11-06 10:49:41 +0000 |
---|---|---|
committer | adam <adam@pkgsrc.org> | 2018-11-06 10:49:41 +0000 |
commit | e95eaa3938da7b7d60ccaf780e08c2bc75d74b41 (patch) | |
tree | a5f5e1df5c9c84380162d21411ab0b8055bb514e /biology | |
parent | 63e327a93abc1fa88aa102b56d0d6bab49bb8fa1 (diff) | |
download | pkgsrc-e95eaa3938da7b7d60ccaf780e08c2bc75d74b41.tar.gz |
samtools: updated to 1.9
Release 1.9:
* Samtools mpileup VCF and BCF output is now deprecated. It is still
functional, but will warn. Please use bcftools mpileup instead.
* Samtools mpileup now handles the '-d' max_depth option differently. There
is no longer an enforced minimum, and '-d 0' is interpreted as limitless
(no maximum - warning this may be slow). The default per-file depth is
now 8000, which matches the value mpileup used to use when processing
a single sample. To get the previous default behaviour use the higher
of 8000 divided by the number of samples across all input files, or 250.
* Samtools stats new features:
- The '--remove-overlaps' option discounts overlapping portions of
templates when computing coverage and mapped base counting.
- When a target file is in use, the number of bases inside the
target is printed and the percentage of target bases with coverage
above a given threshold specified by the '--cov-threshold' option.
- Split base composition and length statistics by first and last reads.
* Samtools faidx new features:
- Now takes long options.
- Now warns about zero-length and truncated sequences due to the
requested range being beyond the end of the sequence.
- Gets a new option (--continue) that allows it to carry on
when a requested sequence was not in the index.
- It is now possible to supply the list of regions to output in a text
file using the new '--region-file' option.
- New '-i' option to make faidx return the reverse complement of
the regions requested.
- faidx now works on FASTQ (returning FASTA) and added a new
fqidx command to index and return FASTQ.
* Samtools collate now has a fast option '-f' that only operates on
primary pairs, dropping secondary and supplementary. It tries to write
pairs to the final output file as soon as both reads have been found.
* Samtools bedcov gets a new '-j' option to make it ignore deletions (D) and
reference skips (N) when computing coverage.
* Small speed up to samtools coordinate sort, by converting it to use
radix sort.
* Samtools idxstats now works on SAM and CRAM files, however this
isn't fast due to some information lacking from indices.
* Compression levels may now be specified with the level=N
output-fmt-option. E.g. with -O bam,level=3.
* Various documentation improvements.
* Bug-fixes:
- Improved error reporting in several places.
- Various test improvements.
- Fixed failures in the multi-region iterator (view -M) when regions
provided via BED files include overlaps
- Samtools stats now counts '=' and 'X' CIGAR operators when
counting mapped bases.
- Samtools stats has fixes for insert size filtering (-m, -i).
- Samtools stats -F now longer negates an earlier -d option.
- Fix samtools stats crash when using a target region.
- Samtools sort now keeps to a single thread when the -@ option is absent.
Previously it would spawn a writer thread, which could cause the CPU
usage to go slightly over 100%.
- Fixed samtools phase '-A' option which was incorrectly defined to take
a parameter.
- Fixed compilation problems when using C_INCLUDE_PATH.
- Fixed --version when built from a Git repository.
- Use noenhanced mode for title in plot-bamstats. Prevents unwanted
interpretation of characters like underscore in gnuplot version 5.
- blast2sam.pl now reports perfect match hits (no indels or mismatches).
- Fixed bug in fasta and fastq subcommands where stdout would not be flushed
correctly if the -0 option was used.
- Fixed invalid memory access in mpileup and depth on alignment records
where the sequence is absent.
Diffstat (limited to 'biology')
-rw-r--r-- | biology/samtools/Makefile | 27 | ||||
-rw-r--r-- | biology/samtools/distinfo | 10 |
2 files changed, 19 insertions, 18 deletions
diff --git a/biology/samtools/Makefile b/biology/samtools/Makefile index acb712e98a9..c4018af7e21 100644 --- a/biology/samtools/Makefile +++ b/biology/samtools/Makefile @@ -1,26 +1,27 @@ -# $NetBSD: Makefile,v 1.2 2018/08/22 09:42:59 wiz Exp $ +# $NetBSD: Makefile,v 1.3 2018/11/06 10:49:41 adam Exp $ -DISTNAME= samtools-1.8 -PKGREVISION= 1 +DISTNAME= samtools-1.9 CATEGORIES= biology MASTER_SITES= ${MASTER_SITE_GITHUB:=samtools/} -GITHUB_TAG= ${PKGVERSION_NOREV} +EXTRACT_SUFX= .tar.bz2 +GITHUB_RELEASE= ${PKGVERSION_NOREV} MAINTAINER= bacon@NetBSD.org HOMEPAGE= http://www.htslib.org/ COMMENT= Tools for manipulating next-generation sequencing data LICENSE= mit -USE_TOOLS+= autoconf automake autoreconf gmake perl:run -GNU_CONFIGURE= yes -REPLACE_PERL= misc/*.pl misc/plot-bamstats test/test.pl -REPLACE_PYTHON= misc/*.py +USE_TOOLS+= gmake perl:run +GNU_CONFIGURE= yes +CONFIGURE_ARGS+= --with-htslib=system +TEST_TARGET= test -pre-configure: - cd ${WRKSRC} && autoreconf +REPLACE_PERL= misc/*.pl +REPLACE_PERL+= misc/plot-bamstats +REPLACE_PERL+= test/test.pl +REPLACE_PYTHON= misc/*.py -.include "../../mk/curses.buildlink3.mk" -.include "../../lang/python/application.mk" .include "../../biology/htslib/buildlink3.mk" -.include "../../devel/zlib/buildlink3.mk" +.include "../../lang/python/application.mk" +.include "../../mk/curses.buildlink3.mk" .include "../../mk/bsd.pkg.mk" diff --git a/biology/samtools/distinfo b/biology/samtools/distinfo index 59523c11fb1..5100c09cb1f 100644 --- a/biology/samtools/distinfo +++ b/biology/samtools/distinfo @@ -1,6 +1,6 @@ -$NetBSD: distinfo,v 1.1 2018/05/07 18:37:31 bacon Exp $ +$NetBSD: distinfo,v 1.2 2018/11/06 10:49:41 adam Exp $ -SHA1 (samtools-1.8.tar.gz) = 5ac70ba4c324c223398ab4c0689933564985303c -RMD160 (samtools-1.8.tar.gz) = 47e4fde82a686669322fd330374aa37b148b7954 -SHA512 (samtools-1.8.tar.gz) = 82e4d5f855f944cc989902154a99eada2b4c81939bd936fa1d471cf9ff01e03c8a7f773928ae55ef691f2827aa4076126678940bb89a322fa56d83c517677d0c -Size (samtools-1.8.tar.gz) = 4047378 bytes +SHA1 (samtools-1.9.tar.bz2) = 267191dc2f5d24eb0b22c0094138270ed5384241 +RMD160 (samtools-1.9.tar.bz2) = b7f49e1bdd569f63ec53330f43df7e5566ba8a16 +SHA512 (samtools-1.9.tar.bz2) = 6c8207754615ae8dad84b278f93e7d57eb40b818efb80f6aff6aba22fde5772247724e86596e765791e3175ffbb683393dd3ddfe7681c73eef4eb4f2342bf68f +Size (samtools-1.9.tar.bz2) = 4440405 bytes |